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EF1a-KRAS-V5/GFP lentiviral vector expressing V5 under EF1a promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Human KRAS ORF cDNA lentivirus (wild type) Lentiviral particles packaged with expression vector for wild type human KRAS proto-oncogene GTPase (KRAS) transcript variant a An EF1 promoter drives expression of KRAS fused to a C-terminal V5 tag and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration allowing for easy establishment of long-term stable cell lines with consistent read out of fluorescent (GFP RFP) or luminescent (firefly luc) reporters
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SMAD 2/3 Responsive Luciferase Reporter NIH/3T3 Stable Cell Line is derived from mouse fibroblast,and stably express firefly luciferase reporter gene under the control of SMAD 2/3 response element. This cell line is an ideal cellular model for monitoring the activation of TGFbeta Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
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